Bio-Works Technologies demonstrated that human polyclonal IgG was captured on a BabyBio A 1 mL column eluted with 100 mM Gly-HCl at pH 2.7 and applied immediately to three BabyBio Dsalt 5 mL columns connected in series.
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Your antibody comes off protein A into acid. ๐๐ก๐๐ง ๐ฐ๐ก๐๐ญ? Protein A elution needs low pH, and acidic pH may induce aggregation of antibodies. ๐๐ฎ๐ซ ๐จ๐ฐ๐ง elutionโฆ
Company
Bio-Works Technologies
Next generation chromatography resins
- Industry
- Biotechnology Research
- Location
- Uppsala, SE
- Company size
- 11โ50 employees
About Bio-Works Technologies
Bio-Works was founded in 2006 and is involved in R&D, manufacturing and supply of innovative, leading edge products used to separate proteins and other biomolecules. Our staff is rich in experience within the Swedish Life science industry and are specialized in resins for separation and purification. Bio-Works'โ research, development and production facilities are located in Uppsala, Sweden. We are actively working with sustainable development and our Quality Management System is based on ISO 9001:2015 standards. Bio-Works produces agarose based products with exceptional performance. These are used in academic research laboratories, biopharmaceutical companies, and many more areas.
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Research & Knowledge
Bio-Works Technologies tested pH 2.7 for elution buffer conditions and found that it triggered the highest level of high molecular weight species.
Research & Knowledge
Bio-Works Technologies demonstrated that 93% of a 1.5 mL sample was removed from a single 5 mL BabyBio column and 82% of a 300 ยตL sample was removed from a 1 mL BabyBio column.
Research & Knowledge
Bio-Works Technologies published a study showing that a WorkBeadsโข 40S capture step reduced impurity load from 27.5% to 12% before the feed ever reached the reversed phase step, achieving 99.7% purity against a 99% target at 90% yield.
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